University of Minnesota LRB/MTRF - Lions Research Bldg/McGuire Translational Research Facility Room 1-110 2001-6th St SE Minneapolis, MN 55455 Monday, October 19, 2009 2:30-6:00PM (local time) Presented in conjunction with The National Institute for Pharmaceutical Technology and Education (NIPTE) and with the support of the American Institute of Chemical Engineers (AIChE) Developed in partnership with Amgen and Pfizer, Inc., the ALA Spotlight Series continues in 2009 with an educational, scientific-based program on the picturesque campus of the University of Minnesota. There is no cost for attendees — Registration for this event has been underwritten by ALA, Amgen and Pfizer, Inc. This is an excellent learning and networking opportunity for post-doctoral associates, graduate students, under graduate students, academicians and government personnel, researchers, engineers, and business professionals interested in laboratory automation and technologies for early-stage science focused on drug discovery and development. ALA Spotlight Series Program 2:30-3:00 p.m. Automated work flows for Small Molecule Synthesis & Purification at Amgen Peter Grandsard, Chemistry Research & Discovery (CR&D), Amgen Inc., Thousand Oaks, CA. This presentation will offer an overview of the automation approaches and instrumentation used or researched at Amgen for synthesis of small molecules at different scale as well as subsequent purifications. We synthesize chemical matter in batch reactors and in continuous or segmented flow setups. Reactions performed in flow benefit from small reaction volumes, rapid efficient mixing, a large surface area to volume ratio, excellent heat conduction, and the ease of reaction scale up from grams to metric tons. We have evaluated flow-based systems from several commercial and non-profit technology providers, as well as a number of in-house-built devices. These evaluation studies involved bromination reactions, ozonolysis, cyclopropanation, hydrogenation, and tetrazole formation. In this presentation some of the results of these efforts will be discussed. In Research, most small molecules are synthesized to be screened for biological activity, and thus need to be of high purity to provide reliable screening results. In the second part of this presentation we will describe CR&D's high throughput mass-directed purification process. This very integrated process takes pre-purification analysis, fraction dry-down, and post-purification reformatting tasks into account. It is based on a one fraction/sample approach, which allows efficient use of fraction collector deck-space and eliminates the need for extensive post-purification fraction handling. We will elaborate on the overall efficiency and capacity of the process and its potential to adapt to the ever changing needs in a discovery setting. 3:00-3:30 p.m. Simcell: A high throughput solution for mammalian process development Sriram Srinivasan, Senior Principal Scientist, Pfizer, Inc. Application of Simcell in Mammalian Upstream Process Development Design of Experiments (DOE) approach has been used in mammalian cell culture process development for a variety of purposes like optimizing growth conditions, increasing productivity and process validation. This approach requires a large number of conditions tested (usually in multiples) which is both time consuming and resource intensive using conventional bioreactor. Hence there is a need to use high throughput systems that can automate the process and evaluate multiple conditions in a single campaign. The high throughput capability of the Simcell can be utilized to perform complex fed-batch experiments across multiple reactors. Parameters like temperature, pH, feed rates can be controlled and cell density, dissolved oxygen can be monitored. In addition using an automated downstream system further analysis like viability, product titer can be measured. Such a platform provides a valuable tool for optimizing process conditions that then can be scaled up in a bioreactor. Similar approach can also be used for screening clones and conditions. In this presentation we would talk about the strategies that we utilize at Pfizer for process development using Simcell. We would present case studies for utilization of Simcell. We would also show that the Simcell data offers a reliable match for the large scale data. 3:45-4:45 p.m. Life After Graduate School & Your First Year on the Job: Presented by the American Institute of Chemical Engineers (AIChE) Presenter: Matt Atkins, AIChE, Large Cap Project Engineer, Fluor Corporation — PES Question & Answer Panel Members: Peter Grandsard, Ph.D., Executive Director Research, Amgen Sriram Srinivasan, Senior Principal Scientist, Pfizer, Inc. This informal roundtable discussion led by early-career professionals new to the workforce is meant to engage participants in discussion about making the transition from their lives as students to their lives as professionals. With participation from Amgen and Pfizer, Inc. this forum is a means for students to ask specific questions about the workforce, job market and what to expect during their job search and first year on the job. 4:45-5:45 p.m. Networking Session with Presenting Scientists From Amgen and Pfizer, Inc. Interact in a social atmosphere with fellow students and industry professionals at this one-hour networking session. Pizza and refreshments provided. Chance to win one of many great prizes, including gift certificates to local establishments and American Express Gift Cards.