ARTEL (Booth 551)
25 Bradley Drive
Westbrook, Maine 04092
+1.207.854.0860 ; 888.406.3463
+1.207.854.0867 fax
www.artel-usa.com
Determining Dilution Accuracy in Microtiter Plate Assays Using the ARTEL Multichannel Verification System (MVS®)
Many critical assays are based upon conducting accurate dilutions of key reagents. Accurately knowing sample concentration is critical for properly interpreting experimental results, which can only be obtained if the experimental dilution ratio is known and controlled. Herein we present an application of the MVS which provides a NIST traceable, dual-dye absorbance method for determining the accuracy of each step in a dilution process. The measurements provided by this method allow for testing both single and multiple point dilutions, and cover a testable dilution range of up to 1/2000. Both theory and experimental validation of this method will be discussed.
Beckman Coulter, Inc. (Booth 227)
4300 N. Harbor Boulevard
Fullerton, California 92834
800.742.2345; 800.643.4366 fax
www.beckman.com
Automation and Detection of a GPCR Functional Assay
75 Inositol phosphate 1 (IP1) is a direct metabolite of IP3 which accumulates in cells following Gq recepetor activitation and is stable in the presence of LiCl making it an ideal marker for a GPCR (Gq) functional assay. In collaboration with Cisbio, Beckman Coulter has automated the IP-One® HTRF® assay along with detection and analysis using the Beckman Coulter Biomek® Laboratory Automation Workstation and PARADIGM™ Detection Platform. This workshop will discuss the details of the IP-One assay, PARADIGM™ Detection Platform and automation methods and results.
Corning Incorporated (Booth 337)
900 Chelmsford Street - Tower 2, 4th Floor
Lowell, Massachusetts 01851
+1.978.442.2200; 800.492.1110
+1.978.442.2476 fax
www.corning.com/lifesciences
Label-Free High-Throughput Screen of Endogenously Expressed Beta 2 Adrenergic Receptor in Live Human Epidormoid Carcinoma Cells With the Corning Epic® System
In this study, we show how the Corning® Epic® System, which uses an optical biosensor to measure the dynamic mass redistribution (DMR) of cellular contents, can be used to study endogenously expressed G-protein coupled receptors. The DMR measurement is a non-invasive, label-free, and integrated response to cell stimulation. Here we used DMR to study the beta 2 adrenergic receptor in human epidermoid carcinoma (A431) cells. Assay robustness was evaluated using a set of 1,400 compounds and high throughput testing was performed on validation set of 10,000 compounds in two replicates on separate days. The results indicate that the Epic® system is well suited for both high information (kinetic) and high throughput (end-point) operation. Reproducible hits were identified with good quality control, utilizing both single and dual mode reads. This is the largest label-free high-throughput screen reported to date, and the results suggest the great potential for optical biosensors in drug discovery applications.
Eppendorf North America (Booth 151)
One Cantiague Road
Westbury, New York 11590
+1.516.334.7500; 800.645.3050
+1.516.334.7521 fax
www.eppendorf.com
Taking Statistics Seriously: Benefits of Automation in Real-Time PCR
The ability to positively detect a difference in the amount of target nucleic acid by real-time PCR in two samples depends on five factors: (i) the false positive rate, (ii) the false negative rate, (iii) the magnitude of the difference, (iv) the variability in the data, and (v) the number of replicates. These factors are related such that any four determine the fifth. For given false negative and false positive rates one can calculate the number of replicates needed to detect a certain difference in gene expression for a given variability in the data. In my talk I will exemplify how the number of replicates can be reduced (and money saved) by reducing the technical variability in the experimental data by accurate automation of the sample dispensing process compared to manual dispensing.
Labcyte, Inc. (Booth 479)
1190 Borregas Avenue
Sunnyvale, California 94089
+1.408.747.2000; +1.408.747.2010 fax
www.labcyte.com
Assay Optimization and Kinase Profiling in µHTS Format
Successful kinase screening requires precise liquid handling, robust assays, reliable plates, and sensitive detection methods. With the increasing number of options available to the screener, it is a challenge to choose which are best suited for a particular application. This workshop demonstrates the complementarity of diverse technologies for a 1536-well kinase assay application. Using a luminescent kinase technology platform from Promega, we demonstrate the optimization of kinase assays with the Deerac Fluidics Equator dispenser, using assay plates from Corning and with luminescent detection by BMG LABTECH's Pherastar. Following assay optimization, the Labcyte Echo 555 liquid handler is used for ultra low-volume compound dispensing for screening and compound titration applications. Data show that miniaturization does not compromise quality when lower volume assays are required.
PerkinElmer (Booth 427)
940 Winter Street
Waltham, Massachusetts 02451
+1.203.925.4602; 800.762.4000
+1.203-944.4904 fax
www.perkinelmer.com
Automation and Application Partnership Approaches from Screening to Clinical Diagnostics
Having recently received ISO 13485 certification for the JANUS Automated Workstation, we will describe our Integration Solutions team approach to providing application and OEM solutions for your laboratory. We will feature Luminex xMAP data from key application partnerships in molecular diagnostics. ISO 13485 certification is vital for a variety of assay development and screening applications and meets the growing demand from our customers for complete, precise and accurate assay automation.
QIAGEN(Booth 204)
19300 Germantown Road
Germantown, Maryland 20874
+1.240.686.7700; 800.362.7737
+1.240.686.7689 fax
www.qiagen.com
QIAsymphony® SP — Pure performance
QIAGEN's new medium — to high-throughput automated system — the QIAsymphony SP — sets a new milestone in automated sample technologies by combining performance-leading magnetic-particle chemistry with a broad application range for RNA, DNA, and protein purification. Cutting-edge technology, innovative functions, and optimized protocols for up to 96 samples per run, plus processing of large sample volumes give you high-performance results you can trust.
The modular design secures your investment since instrument capabilities can be easily extended to include, for example, assay setup enabling automation of complete workflows.
The QIAsymphony SP is intended for general laboratory use. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Trademarks: QIAGEN®, QIAsymphony® (QIAGEN Group).
Symyx Technologies, Inc. (Booth 351)
3100 Central Expressway
Santa Clara, California 95051
+1.408-764-2000; +1.408-748-0175 fax
www.symyx.com
Automating Chemistry for Accuracy and Efficiency in the Lab
Labs in the Life Sciences industry are facing increasing pressure to run more samples, more efficiently, and make results available more quickly. The use of automation and integration offers the ability to rapidly prepare, process and analyze samples consistently for a variety of pre-formulations, formulations and analytical applications. This talk will highlight Symyx Benchtop Systems and Integrated Workflows which can effectively prepare and workup samples of powders, slurries, solutions, and viscous liquids on one single automation platform using library design, experiment execution and data analysis software. We'll cover lab use cases, accuracy achieved and R&D time saved in effectively automating chemistry processes.
Tecan (Booth 305)
4022 Stirrup Creek Drive - Suite 310
Durham, North Carolina 27703
+1.919.361.5200+1.919.361.5201 fax
www.tecan.com
Innovative Solutions Flow With Tecan Robotics
We invite you to see how Tecan is enhancing laboratory automation in the 21st century. Tecan works to bring ease of use to our products through our hardware, software interfaces, and standardized solutions.
Thermo Scientific (Booth 405, 419)
81 Wyman Street
Waltham, Massachusetts 02451
+1.781.622.1000; 800.365.7587
+1.781.622.1207 fax
www.thermo.com
Introducing Thermo Scientific Momentum-the Can Do Automation Management Software
For those who have struggled with the limitations of traditional automation control software, you'll want to find out what Momentum can do for you!
Momentum takes automation and laboratory workflow management software to the next level. It's been designed to provide easy and flexible user control over a range of complex processes.
Learn about the broad range of advanced capabilities:
- Real-time data driven decision making
- Flexible choice of scheduling and mover technologies
- Instrument — "Hot swap"
- Open topology—communication across multiple movers and multiple systems
- Sample tracking and data integration
- Inventory Managemento Powerful process monitoring and visualization
Join us for a complimentary lunch to explore all the possibilities
Velocity11 (Booth 327)
3565 Haven Avenue
Menlo Park, California 94025
+1.650.846.6600; +1.650.846.6620 fax
Controlled Environments in Laboratory Automation
Environmental control is critical to the successful implementation of a variety of automated systems. The needs range from the low complexity demands of compound management for ambient temperatures and dust protection in low humidity, low temperature chambers, to the sophisticated cell culture environment demanding higher temperatures, stable CO2 environments, and sterility. This workshop will guide you through a variety of approaches Velocity11 has taken to protect our customers' valuable reagents, cells, and compounds. Working with liquid handling stations, workstations, and integrated systems, environmental control can be achieved by integrating with precisely controlled sub-units such as dedicated incubators and compound storage systems or by controlling the environment of entire systems. We will outline both approaches and highlight the role of VWorks® Automation Control software in integrating with external storage and LIMS systems.
ARTEL (Booth 551) and Beckman Coulter, Inc. (Booth 227)
25 Bradley Drive
Westbrook, Maine 04092
+1.207.854.0860; 888.406.3463
+1.207.854.0867 fax
www.artel-usa.com
Understanding Method Transfer Pitfalls: Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation
Assays are usually performed on the benchtop using handheld pipettes before transfer to an automated liquid handler. Automating a manual method may require time, but automation helps lower costs, increase throughput, and potentially avoids errors associated with a manual method. During the transfer, however, the manual assay should be directly compared to the automated assay for consistencies in pipetting performance. In addition, the use of Beckman Coulter manufactured pipette tips can ensure correct pipetting operation. Beckman Coulter and ARTEL jointly discuss the importance of diagnosing liquid handling variability during method transfer because an undetected difference in accuracy will impact the integrity of the assay as the automation process continues.
Beckman Coulter (Booth 227)
4300 N. Harbor Boulevard
Fullerton, California
92835 800.742.2346
800.643.4366 fax
www.beckman.com
and
Genentech, Inc.
1 DNA Way
South San Francisco, California 94080
www.gene.com
Integration of High-Throughput Laboratory Automation or Cell Culture Process Monitoring Technologies
Large scale suspension mammalian cell culture present challenges to the currently existing laboratory automation technology to improve efficiency of bioprocess development. The Biomek® FX Laboratory Automation Workstation was employed to prepare reagents and media in TubeSpin™ tubes and 96-well plates. High-throughput osmometer, flow cytometer, and pH meter were developed and integrated with the Biomek® FX. This system is designed to deliver throughputs at least ten times higher than typical shake flask systems. This workshop will: 1) Introduce automated Liquid Handling in Process Development; 2) Describe configuration of automation with detection technology that improve cell culture process development, and 3) Discuss new software features to enhance sample processing, data tracking, data mining, and data archiving.
Corning Incorporated (Booth 337)
900 Chelmsford Street - Tower 2, 4th Floor
Lowell, Massachusetts 01851
+1.978.442.2200; 800.492.1110
+1.978.442.2476 fax
www.corning.com/lifesciences
Cell Culture Scale Up Solutions Utilizing Automation in Conjunction With High Yield Culture Vessels
The increased demand of high-throughput cell based assay screens has led to scale up challenges in cell culture. These include the need to maintain numerous cell lines simultaneously in culture, the reproducibility of cells throughout screening, and the capacity limitations of cell culture scientists.
The SelecT CompacT from The Automation Partnership along with the HYPERFlask™ Cell Culture Vessel from Corning work together to provide a solution. CompacT SelecT grows cells from multiple cell lines in T-flasks for cell line maintenance and expansion. It can also generate assay-ready plates on demand for cell-based screening and assay development. The HYPERFlask vessel increases the efficiency of cell culture by allowing 1720cm2 of growth area in the footprint of a traditional T175cm2 flask. The HYPERFlask utilizes multiple layers of gas permeable growth surfaces for efficient gas exchange.
Dionex Corporation (Booth 173)
1228 Titan Way 205
Sunnyvale, California 94085
+1.408.737.0700; +1.408.730.9403 fax
www.dionex.com
Advanced Automation Tools for Increased Productivity in the HPLC Laboratory
Recently there has been an increased interest in accelerating HPLC methods, in order to achieve a higher sample throughput. But sometimes changing an established and tested method can be challenging and time-consuming. In this workshop, Dionex will present some newly developed technologies that enable an increase in sample throughput technologies such as SmartStartup/Shutdown, Automated Application Switching, and Parallel/Tandem Mode operation.
IDBS (Booth 238)
2 Occam Ct, Surrey Research Park
Guildford, Surrey, GU2 7QB United Kingdom
+ 011.44.1483.595000; + 011.44.1483.595001 fax
www.idbs.com
Data Management for HTS and HCS Screening
This workshop will aim to provide an understanding of how to improve screening efficiency by utilizing tools available and looking at process and workflow.Aimed at users and scientists familiar with ActivityBase as a screening tool, it will illustrate how to get the best out of your workflow by improving management and creation of screening templates, using the ActivityBase XE Module.
Learn about:
- improving workflow
- reducing time taken to create and maintain analysis templates
- how to improve efficiency in your laboratory
ActivityBase XE provides a single environment for data capture, display, manipulation and verification.
Promega Corporation (Booth 257)
2800 Woods Hollow Road
Madison, Wisconsin 53711
+1.608.274.4330; +1.608.277.2601 fax
www.promega.com
Personal Automation Enhances Nucleic Acid & Recombinant Protein Sample Prep Activity
Up to 16 Samples at a TimeThe Maxwell® 16 System is the only Personal Automation™ system that offers basic research, clinical diagnostic and forensic testing users the ability to purify DNA, RNA and recombinant protein. You can process 1 to 16 samples with consistent, reliable purification in 30-45 minutes.
RTS Life Science Limited (Booth 205)
Northbank, Irlam
Manchester, M44 5AY United Kingdom
+ 011.44.161.7772000; + 011.44.161.7772003 fax
www.rtslifescience.com
and
Labcyte, Inc. (Booth 356)
1190 Borregas
Sunnyvale, California 94089
+1.408.747.2000; +1.408.747.2010 fax
www.labcyte.com
A New Flexible Approach to Compound Library Management Using the Labcyte Echo
The Labcyte Echo gives new opportunities to improve compound library management including: flexibility to allow both plate stamping and hit picking on the same unit and, using 1536 well plates as a compound source.
There have long been discussions regarding the benefits of large integrated systems against a workstation approach. RTS Libradexx is an integrated software and hardware solution that uses the Labcyte Echo to combine the benefits of large integrated compound management systems i.e.: large library management, sample tracking, environmental control and extended hours of operation with those of a workstation approach i.e.: simplicity, flexibility to schedule work across workstations, responsiveness and ease of expansion.
This workshop will provide updates on the latest Labcyte Echo developments as well as an overview of the RTS Libradexx Compound Management System.
Yole Développement (Booth 612)
45 Rue Ste Genevieve
Lyon, France 69006
+33.479.830189
+33.472.830183 fax
What Are The Key Trends in Microfluidics For The Next 5 Years?
We invite you to join us January 28th at the Palm Springs Convention Center, Palm Springs, CA and be a part of our MEMS Market Briefing dedicated to the Microfluidics market and related applications.
Yole Développement will present our marketing analysis of Microfluidic technologies. During this session, you will hear about market, application and technology trends. And also vision of key players through the presentation of an invited speaker Moreover, it will be an open networking place for Microfluidics experts and managers of life science companies.
This meeting is aimed at those who wish to get up-to-date on news and recent developments, understand how diagnostic companies are applying microfluidic technology. Yole Développement has designed this program for Executives (CEO, CTO, Marketing, Commercial, Business Development, R&D Managers…), investors, academics … For more information, please contact Sandrine Leroy (leroy@yole.fr).
